The WI-38 cell line was developed in July
1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months
gestational age. Cells released by trypsin digestion of the lung tissue were used for the
primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal
diploid female. A maximum lifespan of 50 population doublings for this culture was
obtained at the Repository. A thymidine labelling index of 86% was obtained after
recovery. G6PD is isoenzyme type B. This culture of WI-38 is an expansion from passage 9
frozen cells obtained from the submitter.
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Lung Fibroblasts (adult)
All cells are performance tested and test
negative for HIV-I, hepatitis-B & C, mycoplasma, bacteria, yeast and fungi. A
certificate of analysis (CoA) is provided for each cell strain purchased. Cell
Specifications: Clonetics cells are guaranteed to perform as indicated when used with
Clonetics media and reagents.
Typical Properties
- Sterility : Neg.
- Mycoplasma : Neg.
- Hepatitis B by PCR : Neg.
- Hepatitis C by PCR : Neg.
- HIV-1 by PCR : Neg.
Lung Fibroblasts (adult)